Intact Protein Molecular Mass Measurement of Purified Protein Samples Using Intact Protein Mass Spectrometry Analysis Technology
Price: $450 per sample
Turnaround Time: 5 to 10 business days
Intact protein analysis provides information on the accurate mass of the protein and the relative abundance of its isoforms and modifications. It is an invaluable approach for the analysis of biologics throughout manufacturing to assess purity and heterogeneity characteristics for lot release purposes.
Poochon’s intact protein analysis services are designed for intact protein mass measurement to confirm the sequence of a purified protein and the relative abundance of its isoforms, for example a modification with different glycans. Intact protein characterization is performed on samples in solution using a combination of liquid chromatography (LC) and electrospray ionization mass spectrometry (ESI-MS). Purified proteins of molecular weight up to 160 kDa at a minimum amount of 1 µg can be analyzed successfully.
Examples using Intact Protein Analysis:
