In 2017, Poochon Scientific and Quintarabio joined forces to offer the area’s first 24-hour Sanger DNA sequencing service center. Thanks to this partnership, Poochon is able to expeditiously collect, process, and analyze samples for DNA sequencing in order to return results within 24 hours on business days. Through preferred pick-up and various drop box locations, Poochon offers convenient sample submission options for local clients. Quintarabio’s easy ordering platform allows for simple order processing and results.
To register an account and place an order below, you will be redirected to Quintarabio’s website.
Services we provide
About 200 ng plasmid DNA is used in one Sanger sequencing reaction, the maximal volume of plasmid DNA used is 5 ul. We prefer plasmid with minimal concentration of 30 ng/ul.If the plasmid size is more than 20 kb, please double the amount of DNA.
We accept both purified and unpurified PCR samples. If samples are unpurified PCR, we will perform PCR purification before sequencing. For PCR products, we use about 10 ng DNA per kb, so please kindly provide PCR size information when making orders.
Direct colony sequencing services utilizing rolling circle amplification (RCA) or PCR to enable Sanger sequencing without the need for plasmid preparation.
We have to perform PCR amplification before sequencing, please provide PCR primers and expected amplicon size. Please send at least 10 ul DNA sample, with concentration ≥ 30 ng/ul
Premixed for success
Advantages of premixed samples:
Mixed in the same tube: Template + Primer
| 10 μl Template | 5 μl Primer | ||
|---|---|---|---|
| Type & Size | Concentration | Concentration | |
| Plasmid | ds plasmid DNA (≤ 10 kb) | 80 ng/μl | |
| ds plasmid DNA (> 10 kb) | 100 ng/μl | 5 pmol/μl(5 μM) | |
| ds plasmid DNA (< 10 kb) | 50 ng/μl | ||
| Purified PCR | |||
| ≤ 1 kb | 5 ng/μl | ||
| 1 kb - 2 kb | 10 ng/μl | ||
| 2 kb - 3 kb | 15 ng/μl | 5 pmol/μl (5 μM) | |
| 3 kb - 4 kb | 20 ng/μl | ||
| >4 kb | 50 ng/μl | ||
| 10 μl Template | ||
|---|---|---|
| Type & Size | Concentration | |
| Plasmid | ds plasmid DNA (≤ 10 kb) | 80 ng/μl |
| ds plasmid DNA (> 10 kb) | 100 ng/μl | |
| ds plasmid DNA (< 10 kb) | 50 ng/μl | |
| PCR product (purified or unpurified) | ||
| ≤ 1 kb | 5 ng/μl | |
| 1 kb - 2kb | 10 ng/μl | |
| 2 kb - 3 kb | 15 ng/μl | |
| 3 kb - 4 kb | 20 ng/μl | |
| >4 kb | 50 ng/μl | |
Pick a colony and resuspend in diH2O or Tris Buffer
Two sets of tubes:
Tube 1: Colony Suspension
Tube 2: Primer
Pick a single colony with a sterile tip and resuspend in 30ul sterile diH2O or Tris buffer (10mM, ph8.0), 15 ul will be submitted to us, and the remaining 15 ul will be kept by the clients for future uses
Prepare 2 separate tubes: 1 contains 15ul colony suspension, 1 contains 5ul primer at 5pmol/ul
Label the samples with your initials followed by numbers
8-strip PCR tube for samples containing template
Tube 2: Primer
Example: Samples from John Smith
Label on the cap as well as on the side of the tube