Identification of Proteins (Up to 2,000 Proteins) From a Single Gel Band or Gel Spot
Price: $250 per sample
Turnaround Time:5 to 10 business days
Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
Identification of Proteins (Up to 4,000 Proteins) From Any Biological Sample
Price: $450 per sample
Turnaround Time: 5 to 10 business days
The combination of high-performance liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA methods, MS-based technology can identify and quantify protein mixtures simultaneously on a large scale. For interested proteins without an available antibody, MS-based protein identification is the fastest and most reliable approach.
PROMICFINDER, developed at Poochon, is a mass spectrometry-based protein analysis technology capable of identifying and measuring rapidly (high throughput), broadly (thousands of proteins simultaneously), and deeply (high and low-abundance proteins).
The PROMICFINDER Platform is a highly sensitive, quantitative, and reproducible proteomic tool for the profiling of proteins (up to 3,000 different proteins) from a single gel band sample, IP sample, or un-fractioned cell/tissue lysate sample, by a 110 min LC-MS/MS run.
Standardized SOPs for sample preparation and LC-MS/MS analysis ensure the quality and reproducibility of the data. Results are reported in a standardized format ready for generating publishable data.
Quantitative Comparison of the Proteome (Up to 9,000 Proteins) Of 6-18 Samples in One Set of Analysis Using Isobaric Mass Tags
Price: $1200 per sample
Turnaround Time: 15 to 20 business days
Proteome Profiling (Up to 9,000 Proteins per Sample) From Tissue or Cell Line Samples Using Label-Free Quantitation Method
Price: $3000 per sample
Turnaround Time: 15 to 20 business days
Proteome Profiling (Up to 12,000 Proteins per Sample) From Tissue or Cell Line Samples Using the Label-Free Quantitation Method
Price: $4300 per sample
Turnaround Time: 15 to 20 business days
Mass spectrometry is the most powerful tool for proteomics to assess the relative abundance of proteins among biological samples. Numerous methodologies now support relative quantification measurements, providing a routine means to analyze protein expression patterns and post-translational modification states as a function of biological perturbation. Available options include label-free quantification, SILAC (stable isotope labeling by amino acids in cell culture) based quantification, and TMT (tandem mass tag) isobaric multiplex (up to 18plex) labeling quantification.
Poochon developed a standardized platform, PROMICMAPPER, for measuring changes in global protein expression in biological systems upon perturbations using isobaric mass tags and liquid chromatography tandem-mass spectrometry (LC-MS/MS). The PROMICMAPPER platform allows for the quantitative protein profiling of up to 9,000 proteins, or deep quantitative protein profiling of up to 12,000 proteins for a single project.
Additionally, the data generated by PROMICMAPPER can be analyzed using Poochon’s data and pathway analysis tool, PROMICPATH, which uses ten groups of housekeeping protein complexes to evaluate the completeness and variability of a proteome profile. The evaluation ensures the integrity, reliability, and reproducibility of a large set of protein profile data generated by PROMICMAPPER.
PROMICMAPPER is the most cost-efficient approach for identification of differential expression of proteins for elucidating the molecular mechanisms of perturbations and discovery of biomarkers.
Identification of Post-Translational Modifications
Price: $600 per sample
Turnaround Time: 10 to 15 business days
Profiling of S/T/Y Phosphorylation Sites Using a Phosphopeptides Enrichment Approach and Label-Free Quantification Method for Comparison Among Different Samples
Price: $2000 per sample
Turnaround Time: 15 to 20 business days
Quantitative Comparison of the Phosphoproteomes of 6-18 Samples in One Set of Analysis Using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set and Phosphopeptides Enrichment Kit
Price: $1200 per sample
Turnaround Time: 20 to 25 business days
Proteins are subjected to a wide variety of covalent modifications after translation. Many of these post-translational modifications (PTMs) are critical to the protein’s function. The common PTMs include Phosphorylation, Ubiquitination, Methylation, Acetylation and Glycosylation. We have accumulated experiences to develop reliable workflows using available tools and technologies for identifications of PTMs.
Intact Protein Molecular Mass Measurement of Purified Protein Samples Using Intact Protein Mass Spectrometry Analysis Technology
Price: $450 per sample
Turnaround Time: 5 to 10 business days
Intact protein analysis provides information on the accurate mass of the protein and the relative abundance of its isoforms and modifications. It is an invaluable approach for the analysis of biologics throughout manufacturing to assess purity and heterogeneity characteristics for lot release purposes.
Poochon’s intact protein analysis services are designed for intact protein mass measurement to confirm the sequence of a purified protein and the relative abundance of its isoforms, for example a modification with different glycans. Intact protein characterization is performed on samples in solution using a combination of liquid chromatography (LC) and electrospray ionization mass spectrometry (ESI-MS). Purified proteins of molecular weight up to 160 kDa at a minimum amount of 1 µg can be analyzed successfully.
Examples using Intact Protein Analysis:
Protein Sequence Confirmation of a Test Protein Product Compared to a Reference Standard
Price: $1600 per sample
Turnaround Time: 5 to 10 business days
Peptide mapping, an identity test for proteins, is an essential analytical method for elucidating the primary amino acid structure of proteins. For recombinant protein pharmaceuticals, such as monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), peptide mapping is used for proof of identity, primary structural characterization, and quality assurance (QA). Regulatory agencies issued ICH Q6B guidance which specifies the use of peptide mapping as a critical quality test procedure for drug characterization used to confirm desired product structure for lot release purposes. Peptide mapping involves enzymatic treatment of a protein, resulting in the formation of peptide fragments, followed by separation and identification of the resultant fragments by LC-MS/MS in a reproducible manner. It can identify single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations.
Poochon developed a standardized platform for peptide mapping of purified proteins using protease digestion and liquid chromatography tandem-mass spectrometry (LC-MS/MS).
Evaluation of Purified or Partially Purified Protein Samples Including Therapeutics Products at the Early Stage of Development
Price: $600 per sample
Turnaround Time: 5 to 10 business days
Confirmation of Full Amino Acid Sequence (>95% Sequence Coverage) of Any Purified Protein Sample With a Known Target Protein Amino Acid Sequence
Price: $1,200 per sample (for de novo sequencing, please contact us for a quote)
Turnaround Time: 10 to 15 business days
Identification of N-Linked Glycosylation Sites From Purified Protein Samples
Price: $1800 per sample (for O-linked glycosylation, please contact us for a quote)
Turnaround Time: 10 to 15 business days
Characterization of the Profile of N-Linked Glycans Released From Purified mAb Samples Using Rapifluor-Ms™ N-Glycan Kit and LC-MS/MS
Price: $5000 per sample
Turnaround Time: 20 to 35 business days
Poochon developed a robust approach using a range of state-of- the-art orthogonal methodologies to elucidate the primary amino acid sequence, post-translational modifications, and associated micro-heterogeneity and glycosylation of therapeutic products.
Identification of Differentially Expressed Proteins From Two Groups of Samples
Price: 100
Turnaround Time: 5 to 10 business days
Biomarkers, such as proteins, are naturally occurring biomolecules useful for measuring the prognosis and/or progress of diseases and therapies. They are normally present in small amounts in blood or tissue. When the amounts of these molecules change, they may indicate disease. Poochon developed a reliable platform, PROMICMAPPER, and a novel proteomic pathway analysis tool, PROMICPATH, for quantitative global protein profiling and proteomic pathway analysis, which is an ideal combination to differentiate diseased and normal samples for biomarker discovery.
Bioinformatic Analysis of Large Proteomics Datasets and Identification of Differential Expression of Proteins and Involved Pathways
Price: $1800 per data set of a paired group
Turnaround Time: 10 to 15 business days
Biological information flowing from the genome to proteome automatically regulates living cell status in response to internal or external signals. The genome encoding the proteome is relatively static, while the proteome is much more dynamic allowing the protein to orchestrate all biological processes precisely, ranging from central metabolism to cell structure, maintenance, and replication. It is speculated that all biological processes (on/off) are regulated and operated by the dynamics of proteins, such as modifications and changes in abundance. The regulation of different cellular functions has been categorized into a number of pathways, such as the Wnt signaling pathway and the TGF signaling pathway. In each pathway, the components are generally named according to their function, including ligands, receptors, activating regulators, inhibitory regulators, and effectors. To measure the activation strength of a pathway, the protein molecules that belong to ligands, receptors, activating regulators, or inhibitory regulators are grouped as the pathway protein ontology chain (POC), and their relative abundances (ppm) are summed. Based on the summed abundance of each POC, the activation strength or activation status of a pathway can be compared between two proteome profiles.
Poochon developed a novel pathway tool called PROMICPATH using algorithms for protein abundance variation and protein function grouping based pathway analysis. PROMICPATH allows for customized pathway analysis, as well as common pathway analysis, which analyzes 42 common pathways. To ensure data integrity and reliability, PROMICPATH uses 10 groups of 400 housekeeping proteins to evaluate the completeness and variability of a proteome profile.
Price: $100 per sample
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Price: $100 per sample
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Price: $150 per sample
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Price: $200 per sample
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Price: $300 per sample
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Price: $30 per sample
Turnaround Time: 3 business days
Service Description: Preparation of protein lysate from cell pellet
Sample Type: Fresh frozen cell pellet (profiling > 1×106 cells/sample)
Services Include: Sample preparation, sonication, and Pierce™ BCA Protein Assay
Price: $50 per sample
Turnaround time: 3 business days
Service Description: Preparation of protein lysate from tissue sample
Sample Type: Fresh frozen tissue (> 10 mg/sample)
Services Include: Sample preparation, homogenization, sonication, and Pierce™ BCA Protein Assay
Price: 23
Turnaround Time: 7 business days
Service Description: TMT labeling up to 18 plex using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set
Sample Type: Frozen lysate, frozen cell pellet, or frozen tissue
Services Include: Protein lysate preparation, Pierce™ BCA Protein Assay, enzymatic digestion, TMT labeling, and bRP-HPLC Fractionation
Price: $1000 per set
Turnaround Time: 7 business days
Service Description: Basic reversed-phase HPLC fractionation of peptides (TMT/iTRAQ/SILAC/label-free)
Sample Type: Digested peptides mixture, labeled with TMT/iTRAQ/SILAC, or label-free
Services Include: Fractionation by basic reversed-phase HPLC using a C18 column, 150 min LC run collecting elutes into 96 wells, and combination of elutes to 24 or 12 fractions
Poochon conveniently offers a variety of basic services to help complete your project. Whether you only need analysis of a peptide mixture, or simply for us to make cell lysates and measure the concentration, our itemized services will save you time and money. If a full-service package is not right for you, our itemized service options may meet your needs.
Any biological sample can be analyzed. Typical sample types include, but are not limited to: primary cells, cultured cells, PBMCs, tissues, protein mixture samples (e.g. IP and pull-down), fraction of cellular organelles (e.g. exosomes and membrane), body fluids (e.g. plasma/serum, CSF, urine, sputum, saliva, etc.), and FFPE tissue blocks/sections.
Client Designs the Project Workflow and Specifies the Deliverables
Price: Contact us for a quote!
Turnaround Time: Varies
Client Provides the Project Goals and Requirements; Poochon Designs the Workflow and Specifies the Deliverables
Since each of our clients’ projects are unique and require special attention, many of our projects are Poochon designed. We welcome our clients to contact us for a free consultation as we are dedicated to fully understanding project needs and provide expert input in order to deliver a comprehensive proposal!
Price: Contact us for a quote!
Turnaround Time: Varies
Modern proteomics projects can be extremely diverse, creating a need that may not be satisfied under our primary service offerings. In response, Poochon created the customized project option to take advantage of our state-of-the-art instrumentation and expert analytics when a project falls outside our routine offerings.
Any biological sample can be analyzed. Typical sample types include, but are not limited to: primary cells, cultured cells, PBMCs, tissues, protein mixture samples (e.g. IP and pull-down), fraction of cellular organelles (e.g. exosomes and membrane), body fluids (e.g. plasma/serum, CSF, urine, sputum, saliva, etc.), and FFPE tissue blocks/sections.
